Constructing Physical and Genomic Maps for Puccinia striiformis f. sp. tritici, the Wheat Stripe Rust Pathogen, by Comparing Its EST Sequences to the Genomic Sequence of P. graminis f. sp. tritici, the Wheat Stem Rust Pathogen

Table 2

Homology of Pst and Pgt genes. The total numbers of Puccinia striiformis f. sp. tritici (Pst) unigene ESTs from three cDNA libraries compared with and the numbers and percentages of ESTs with significant sequence homology with the whole genomic sequences of P. graminis f. sp. tritici (Pgt).

Pst cDNA library	No. of unigenes	No. of unigenes with Significant	Percentage (%) of unigenes with
		Homology to Pgt genesa	significant Homology to Pgt genesa
Urediniospores	1,306	712	54.51
Germinated urediniospores	869	441	51.21
Haustoria	2,044	279	13.65
Total	4,219	1,432	33.94

a The E value of 1×10⁻⁵ was used as a cut point to determine significant homology.