A Comprehensive Bioinformatics Analysis of the Nudix Superfamily in Arabidopsis thaliana
Figure 4
Conserved motifs in Arabidopsis Nudix hydrolases. (a) Different colored boxes and their designated numbers indicate separate and distinct motifs of 23 of the 25 Nudix hydrolases identified using MEME [23]. The At4g25440 and At1g79690 gene products were omitted from the figure for convenience of presentation. (b) Comparison of the homology model of the At1g30110 protein with that of the structure of the lupin hydrolase. The structure of the At1g30110 gene product (Left), a putative A hydrolase from A. thaliana modeled against the known structure of the lupin Ap4A hydrolase (PDB ID-1JKN) in a complex with ATP-Mg (Right). Motif 4 is colored orange in the homology model, and helices 3 and 4 are marked. The loop region which contains the metal-binding Glu-125 in the lupin enzyme structure, the equivalent loop in the At1g30110 homology model, and the side chain of analogous Glu-126 are also depicted. The catalytic helix containing part of the conserved Nudix box is shown in green in each model.
