International Journal of Genomics

Research Article

Factors Affecting Splicing Strength of Yeast Genes

Table 7

Testing the predictions that introns in highly expressed genes have higher PWMS and smaller variance in PWMS than in lowly expressed genes, with gene expression measured by CAI, mRNA, and protein abundance. Introns spliced by nonspliceosomal mechanisms are excluded. Mann-Whitney tests generate similar results. All tests are two tailed. The results are nearly identical when mRNA abundance from microarray [27] is used instead of that from GATC-PCR [28] or when protein abundance [29] is used instead of the protein synthesis rate [30].


	5′ ss			3′ ss
	CAI	lnMRNA⁽¹⁾	lnPROT⁽²⁾	CAI	lnMRNA	lnPROT

N⁽³⁾	91	48	55	100	53	67
MeanH⁽⁴⁾	11.4927	11.3128	11.4879	8.7188	8.5447	8.6004
MeanL⁽⁵⁾	9.4135	9.7143	9.7581	5.1109	4.9729	5.3359
DF⁽⁶⁾	113	59	55	155	83	82
	4.1635	2.2501	2.2411	9.7833	6.9719	5.7687
	0.0001	0.0282	0.0291	<0.0001	<0.0001	<0.0001

VarH⁽⁷⁾	3.2053	2.8657	2.7345	2.6326	3.4395	3.4220
VarL⁽⁸⁾	10.3934	21.3602	24.6692	20.0609	10.4712	9.9223
	3.2429	7.4537	9.0214	7.6211	3.0444	2.9000
	<0.0001	<0.0001	<0.0001	<0.0001	<0.0001	0.0001

⁽¹⁾Natural logarithm of mRNA abundance [28].
⁽²⁾Natural logarithm of protein synthesis rate [30].
⁽³⁾Number of ss in the highly expressed and lowly expressed groups (note that ).
⁽⁴⁾Mean PWMS in highly expressed group.
⁽⁵⁾Mean PWMS in lowly expressed group.
⁽⁶⁾The -test assuming unequal variance is used. SoDF is not equal to ().
⁽⁷⁾Variance in the highly expressed group.
⁽⁸⁾Variance in the lowly expressed group.