Molecular and Biological Characterization of Chinese Sacbrood Virus LN Isolate
Table 1
Synthetic oligonucleotides for amplification of CSBV genome.
Primersa
Sequence (5′ to 3′)
Nucleotide positionsb
S1 F
5′-GACCCGTTTTCTTGTGAGTTTTAG-3′
41–64
S1 R
5′-GTGTAGCGTCCCCCTGAATAGAT-3′
611–633
S2 F
5′-TATTCAGGGGGACGCTACAC-3′
614–633
S2 R
5′-TATTCCATCGGGGTTATTTG-3′
1713–1732
S3 F
5′-GGAGACGCGCATGGTAAAGA-3′
1644–1663
S3 R
5′-GCGCGGTAAATAAACACTCG-3′
2365–2384
S4 F
5′-ATGGGGGTAAGGGACAATCTG-3′
2290–2310
S4 R
5′-TGCTCTAACCTCGCATCAAC-3′
3423–3442
S5 F
5′-TTACGGGAGCAGCACAACA-3′
3391–3409
S5 R
5′-ATTTCCGATTTACCGATACC-3′
4287–4306
S6 F
5′-CGGTGCGTTATGAACCTTTT-3′
4243–4262
S6 R
5′-AATGCGTAGATTGAGGTGCC-3′
5333–5352
S7 F
5′-GCGCAACTGGCACCTCAAT-3′
5325–5343
S7 R
5′-TTCCAAATATACTTCCCACTGC-3′
6249–6270
S8 F
5′-GTGACGGCAGTGGGAAGTAT-3′
6262–6243
S8 R
5′-GCAGCCTCCTCAGGTGTTAGT-3′
7454–7474
S9 F
5′-TTTGGTAGCGGGGTGTAAG-3′
7322–7340
S9 R
5′-CATTGCGTGGTATCATT-3′
8501–8517
S10 F
5′-TACGAATCGTGATTCGAT-3′
1–18
S10 R
5′-TAAACAAATCGGTATAAGAGTCC-3′
379–401
aF: forward; R: reverse.
bNucleotide positions of S1–S9 primer pairs refer to the published CSBV sequence [7] (Genbank accession no. AF469603); nucleotide positions of S10 primer pairs refer to the published CSBV sequence [10] (Genbank accession no. AF092924.1).
