Differential Protein Expression in Sugarcane during Sugarcane-Sporisorium scitamineum Interaction Revealed by 2-DE and MALDI-TOF-TOF/MS
Table 1
Identification of differentially expressed proteins in sugarcane under S. scitamineum stress.
Spot
Accession no.
Homologous protein
pI
Mr (Da)
Species
Expression
NO. 1
gi73970162
Echinoderm-microtubule-associated protein
8.46
233439.8
Z. mays
+
NO. 2
gi3914465
Photosystem I reaction center subunit VI
10.1
14920.1
Z. mays
NO. 3
gi11134057
Oxygen-evolving enhancer protein 3-1
9.77
23118.5
P. sativum
NO. 4
gi46109064
Unknown
4.7
84190.8
O. sativa
NO. 5
gi8996050
Protective antigen
6.02
62682
A. thaliana
NO. 6
gi66821923
Ribulose-1,5-bisphosphate carboxylase
5.95
48907.6
O. sativa
NO. 7
gi6578770
Rubisco large subunit
6.3
48203.2
L. cuneifolia
NO. 8
gi89347149
2-oxo-acid dehydrogenase E1 component
5.79
95280.7
S. bicolor
NO. 9
gi37780998
Chlorophyll-a/b-binding protein
7.03
18027.3
V. vinifera
NO. 10
gi78777782
Cytochrome-c peroxidase
8.39
45113
Z. mays
+
NO. 11
gi27883935
Ribulose-1,5-bisphosphate carboxylase
5.98
20501.5
C. erectus
NO. 12
gi75817913
Predicted oxidoreductases
6.73
11799
A. thaliana
NO. 13
gi62734371
Abscisic acid and stress-induced protein
6.2
15455.5
O. sativa
NO. 14
gi548603
Photosystem I reaction center subunit II
9.81
21919.4
H. vulgare
NO. 15
gi51459711
DnaK-like chaperone protein HscA
5.56
65281.9
Z. mays
+
NO. 16
gi6691487
Oxygen-evolving complex of photosystem II
8.61
28121.2
C. sativus
NO. 17
gi50508582
Rieske Fe-S precursor protein
8.54
23869.2
O. sativa
NO. 18
gi35211352
Unknown
11.42
43495.3
G. violaceus
NO. 19
gi30408003
NBS-type resistance protein
9.73
19422.6
M. esculenta
NO. 20
gi67004037
Unknown
5.48
155305.5
O. sativa
Notes: Expression ratio was calculated relative to protein level in control sample. The “” indicated that the amount of protein expressed in treatment sample was greater than that in control sample; conversely, “” indicated that the protein expression was downregulated under S. scitamineum stress. The “+” indicated it was a newly induced protein after S. scitamineum stress.
