Generation and Analysis of Expressed Sequence Tags from Chimonanthus praecox (Wintersweet) Flowers for Discovering Stress-Responsive and Floral Development-Related Genes

Table 1

Forward (F) and reverse (R) primers used in real-time PCR.

Gene code	Annotation (sequence homology)	Primer sequence (5′→3′)	Tm (°C) a	Cycles	Product length
Cp88	Membrane channel protein	F: ATGTGGACTTTTTGCTGGAGTTTTT	59	40	90
		R: GCAATACAAGCATTTACCCAGTCCT
Cp173	Low temperature and salt responsive protein	F: CTGACGCTCTTTGGATGGCTACC	59	40	172
		R: ACGAATCAACGGTCACAAACACG
Cp215	Putative abscisic acid-induced protein	F: TGCTCTTTACTTGCTGGCTCGTCT	59	40	193
		R: TCTCGCCCATTCTCTTCTATGTATTTC
Cp274	Glutathione S-transferase	F: ATGTGCTAAATCTCATTCCAGTCTTCC	59	40	85
		R: GCCGTGATATCCTGCGAAACTAG
Cp359	1,4-Alpha-glucan-maltohydrolase	F: TATTATCTGGCAAGGTTCCACTCGTT	59	40	86
		R: TGTGTTGTAGAACCCAGCAGTCAGC
Cp364	Cold acclimation Protein WCOR413-like protein beta form	F: TCCAATAAGCCGAATCAAAATACAGT	59	40	84
		R: GTCTGTCTTCATCGCCAAATACTCC
Cp375	3-Oxoacyl-[acyl-carrier-protein] synthase	F: GTAACTGCGCCGAAACGAGAAAAAG	59	40	78
		R: CCCAAAGACAGAAACCAGCCCC
Cp440	Putative multiple stress-responsive zinc-finger protein	F: GTTTCCCGATTTTGTGTGGCG	59	40	183
		R: AGTTGTTGATGCAGAGAATTGGTCCT
Cp465	Catalase	F: CATCGTTCGCTTCTCTACCGTTATT	59	40	118
		R: TGTTTCCGACCAGATCAAAGTTACC
Cp197	AGL9.2	F: TGAGAATAAGATAAATCGGCAGGTGAC	59	40	128
		R: TTTTCCTCTGCTGGAGAAGACAACG
Cp203	LLP-B3 protein	F: GCGATGTGAAACTTGTCAGTAGCCC	59	40	171
		R: AGTTTGGCACAATCAGGACGAGGTT
Cp268	Caffeoyl-CoA O-methyl-transferase-like protein	F: AACACAAATGGAGAAGAGAAAACCC	59	40	193
		R: CATCGGCAGAGGTCGTCATAAGA
Cp297	SRG1-like protein	F: TAAGAACACCGTCCCATCTCGCTAC	59	40	143
		R: GAGTGCAGTCTCTCCAATTCATCAG
Cp328	STYLOSA protein	F: GGGGAACCAACAAGAACAACAGAT	59	40	161
		R: GCCCAGTGGCACCATTTAGAAGAT
Cp330	RAC-like G-protein Rac1	F: ACCGCTTCTACCGCCTTTTCTCC	59	40	166
		R: AGGTCTTTCCAACAGCACCATCTCC
Cp360	Peroxisomal fatty acid betao-xidation multifunctional protein AIM1	F: TATGCTGGTGTTTTGAAAGAGGCG	59	40	193
		R: CTATGCCAGACCCCATCAAACCTC
Cp383	Secondary cell wall-related glycosyltransferase family 47	F: CAGGCGACACCCCCTCCTCTAAC	59	40	150
		R: TCAAGGCATCAGAGTTACGCACAAAG
Cp423	Putative polyketide synthase	F: GAGTTCAGACACTGTATGCCCTTGG	59	40	165
		R: TTGCTTGTAAAATTCGTCCTTCGTT
Cp436	Allergen-like protein BRSn20	F: CTCTTATCGCTCTCTGCTTCCTTCC	59	40	176
		R: GTTGCTGTTAACGTCTCTGCATTCC
Cp458	MADS-box protein 9	F: TGGTTGAGTATGATGTAGAGAAGCGAC	59	40	98
		R: ACCCATCATCTGAAGGTGTGCTATT
Cp82	Dormancy related protein	F: TGGAATAGAAAGAGCAAATACAGCG	59	40	172
		R: TACAAAAGGCTCAATGGCGTCC
Cp24	No hits	F: GCAGTTTACATACTACGGGAGAGGCT	59	40	106
		R: CGGCTTACGGAATCGTCATCAC
Cp64	Putative protein	F: CCAATCACTCTCCCTGAGGATGTAT	59	40	159
		R: TTCACCGACTCCTTGTTCTTTTAGC
Internal control	Actin	F: GTTATGGTTGGGATGGGACAGAAAG	59	40	199
		R: GGGCTTCAGTAAGGAAACAGGA
Internal control	Tublin	F: TAGTGACAAGACAGTAGGTGGAGGT	59	40	139
		R: GTAGGTTCCAGTCCTCACTTCATC

^aAnnealing temperature.