Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies

Table 1

Summary of output from 454 sequencing (full dataset) and statistics from the full data de novo assemblies.

	Normalised	Unnormalised
Number of raw reads	191312	287576
Number of reads after trimming	145939	247783
Percent of reads retained	76.3	86.2
Mean read length after trimming	290	322
Number of contigs in assembly	5563	2703
Mean contig length (bp)	660	865
Maximum contig length (bp)	1412	4382