Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies

Table 2

Summary of statistics (means with standard errors estimated through jackknifing) of contigs produced by de novo assembly of 454-sequencing reads from normalised and unnormalised transcriptome libraries.

	Blood		Spleen
	Normalised	Unnormalised	Normalised	Unnormalised
Number of sampled reads	29500 (0)	29500 (0)	45800 (0)	45800 (0)
Number of assembled reads	13998 (89.0)	20703 (25.9)	15980 (29.6)	22487 (23.3)
Number of contigs in assembly	3221 (6.1)	1240 (7.4)	3487 (12.8)	2078 (10.6)
Mean contig length	413.7 (0.8)	501.6 (1.8)	385.7 (0.6)	455.2 (1.2)
Maximum contig length	2014 (105)	4777 (240)	1428 (15)	3117 (54)
Mean number of reads per contig	4.7 (0)	13.4 (0.2)	4.7 (0.05)	14.2 (0.3)
Maximum number of reads per contig	48 (1.1)	1944 (108.2)	230 (3.8)	1944 (165.8)
Number of transcripts detected	1184 (5.3)	586 (14.7)	1238 (5.9)	924 (6.0)
Mean number of reads per transcript	7.1 (0.04)	23.9 (0.6)	6.6 (0.03)	22.0 (0.5)