Transcriptional Regulations on the Low-Temperature-Induced Floral Transition in an Orchidaceae Species, Dendrobium nobile: An Expressed Sequence Tags Analysis
Table 1
Summary of the four libraries.
Library name
0 d
(5+10)d
(20+30)d
40 d
Sum**
Total EST
3391
3369
4112
4145
15017
a*Singlet
2704 (79.74%)
2736 (81.21%)
3166 (77%)
3310 (79.86%)
aContig
272
247
325
338
aUnigene
2976 (87.76%)
2983 (88.54%)
3491 (84.9%)
3648 (88%)
Mean length of EST (bp)
654
615
588
745
BlastX against nonredundance protein database (Nr) at NCBI
No hit aUnigene
429
565
770
1477
3241
Hit aUnigene
2547
2418
2721
2171
m*Unigene§
2426
2520
2985
3112
mSinglet
2016
2145
2539
2657
mContig
410
375
446
455
mUnigene§§ (Nonredundant across libraries)
6375
mSinglet
5290 (82.98%)
mContig
1085 (17.02%)
Total mUnigene
9616 (sum of 6375 and 3241)
Blast X against TAIR9 protein datasets at TAIR
No hit aUnigene
651
812
1032
2087
Hit aUnigene
2325 (78%)
2171 (72%)
2459 (70%)
1561 (42%)
mUnigene§
1599
1553
1756
1163
GO annotated mUnigene§
1433
1403
1596
997
*“a” is the first letter of “assembly,” indicating the sequence is generated from assembly using Phrap. “m” is that of “manual,” indicating the sequence is generated from manually removing the redundancies.
**This column lists simple sum of the 4 libraries. § and §§ The nonredundant Unigene in the row marked “§” are nonredundant within a library but maybe redundant across libraries, while those marked “§§” are nonredundant across libraries.
