Gemi: PCR Primers Prediction from Multiple Alignments

Figure 1

Screenshot of the main window of Gemi. (1) Switch from DNA to RNA, (2) browse for the input FASTA file, (3) save the output tabulated text file, (4) the accession numbers and the percentage of the conservation in the consensus will appear in this area, (5) the consensus sequence is written in this area, (6) switch between the options, (7) choose to design probes by ticking real-time PCR, the parameters can be edited in this menu, (8) finally, click to design the primers, and (9) the program progress will be seen in the status bar.