Comparative Analysis of MicroRNAs between Sporophyte and Gametophyte of Porphyra yezoensis

Table 3

Characteristics of P. yezoensis pre-miRNA sequences.

Id	Mfe^a	Length^b	seq^c
Pye-miR1	−104	227	ACGCGCACAGCCTCGCCAACCGCGACGTCCGCCATCGCCA
			CGACCGCCCCCGTCGACAGCTCAACGGTGGCGGCGGCGGG
			GAAGCACGCCGGGTTGTTGTCCGCGGCCGTGCCGTTGGCC
			CCCTCCGCGACATCGCCCGACTTGATCGCCGCGCCCGTGT
			ACACGCACAGCGGGTTGTCCACCTGTGAGATGGGGTTGGA
			CTCGATGGTGTAGGTGAGAAGGTGGCG
Pye-miR2	−34.9	76	GAAAACTCAGGACAGCGACGACGACGACGACCGCAAGCCG
			CGCCCGTCGCCGTCGTACTAGCTGGGCTGATGACAA
			CGTGTGCCGCCGTACATCCGAAGTCATCGAGCTGCAGTTCC
			AGGACATCTTCGTGCTGGCGGACGGCGCCTTCTCCATCCAC
Pye-miR3	−72	193	GTCAACCGTTACAAGAACACGGAGGGACGTGACGACCCTC
			GCCGCCTGGTGTACACCATTCCACGTGACCCCATGCTGGTG
			CACGACCCGGTCCTGGCATTGCTGCGCACC
Pye-miR4	−28.8	47	GGCGACGGCGACGGCGGCGACGCGGCCGCCGACCGATGGC
			TGTCGTA
Pye-miR5	−74.4	138	TAGGCCTGCAGCGTCAGGGCGGGGTGTGTCGCGCCCCCCG
			CGGCACAAAAGCCGCCGTCCGCGGCTAGCGGGAGGGTGGC
			CGGCTTCGAAAGAGGCGTTGCTGAGCGCGGGCGCGCAGAA
			GACTCGTTCGGCGGCCGC
Pye-miR6	−71.2	134	TGGCAGCAGCGCCGAGGGCGATCGCCGCGCGGCCCACCGC
			CGACCCCCCCAGCTCCCCCCTCCGCCCGGCGACGATGGGC
			GTGGGCGTCAGCGGCGGCCGGTGGAGGCTGGAGCGGTCAAC
			GCCCGGGCACGCC
			GCCACCGCCGTGGTGTAGGTGCTGGCACGGACGCCGCCAAC
			GACGATATCCCCCTGCAGCGTCTGGGGGTTGTACAACCCCG
			CCGAGGTGCCCGTGGAGACGGCGGTCACCACCGACGACGC
Pye-miR7	−135	246	GGCGTCGGCGGCCACGTCCAGCGCGTCGCCCACCCGCACC
			GACCGCAGCGGCGCCGCGCGGCCATTGATGTACACCAGGT
			GCCCCGGCGTGGCGGTGAGCGCGTGGCCGCTGCGGGTGGT
			GGCG
Pye-miR8	−117	226	GCGCGCCCCGTCGGACGGGACGAGGGCAGCAAGCCGGCGC
			TTATGGCCGCGCGCGGCCTCCTGCGTGTGCTGCGTCGGGG
			AGAGCGGCTCGAGGTCGCCGGCGAGCCTCGTCTGCATGGC
			CGCCAGCTCGTGCTTTGGGCATGCCCACTTGGGCACGATG
			CCGGAGCGCACCTTCACCTTCAGCGTGTCAGCCGCGTGCC
			CTTTCTCGTCGCAGGCGCCGCACGCT
Pye-miR9	−20.3	71	TGGTGGGTTGTTTCTCTGTGTGTTCTGGGTGCTACGCGCC
			TAAGGTACGTAAAACCACTACACCCCTTCCT
Pye-miR10	−62.2	153	GTGGCTGGTACACAACAAGTACACGCGCTCTGAGATGGG
			CCGGAAGGCAGTCCGTGCCGGCGTCAAGGCCATGTACGC
			GTACCTCGGCGTCACTGACCGCGAGCGCGATGACGACGT
			CGGCAGTGGGACTGTCCTTGGCATCTCTATTGGCCA
Pye-miR11	−79.4	141	ATCCTTGGCCGCCTCGGTGAGGGCTCGGACCTGGTCCAAG
			GCCTTAGCGAGCTTACTCTGGAGGTCAGAGATGACCTCCG
			CCTGGGTGGGGTCGTCCCCCCGCCCAGAGTCTGAGCCCAT
			CCCTTGCGTGGACGCAAGGAA
Pye-miR12	−38.8	79	AAAAGCCGCCTGGGGATGGGCATTGAAGGCGTCCGCGGCT
			TCCTGAAAGCGCTTTGTGCGCGTACCCCGCGCGGCCACA
			GCTGTCGCGTCACAGCTCCAGCGCGCGTGCGCGGGCCGCG
Pye-miR13	−51.2	83	ACGGCCGCGGCCTGTCATGGTCGCTTGGGCACTGATGCGG
			TGG
Pye-miR14	−24.2	72	CATTGCCAACCGCTTCGTGACTCTCGGCATGGGAGAGAGCC
			GCGATTATGGATTGCGGAGAGAGGACAGTGG

^aMinimum free energy (cal/mol) of pre-miRNAs, predicted by mfold.
^bLength of pre-miRNAs.
^cSequence of pre-miRNAs, the mature miRNA was indicated in italics.