Identification and Characterization of MicroRNAs in Macaca fascicularis by EST Analysis

Table 1

Novel miRNAs identified in Macaca fascicularis.

miRNA	Homolog miRNA	EST name	miRNA sequence (5^′-3^′)	Position	NM/nt	LM/nt	LP/nt	A + U(%)	MFE	MFEI
miR-122	hsa	BB880656	UGGAGUGUGACAAUGGUGUUUG	176–155	0	22	56	62.50	−24.2	1.15
	gga	BB891562	UGGAGUGUGACAAUGGUGUUUGU	174–152	0	23	56	62.50	−24.2	1.15
	mdo	BB891562	UGGAGUGUGACAAUGGUGUUUGUGU	174–150	0	25	57	62.50	−24.2	1.13
miR-122^*	hsa	BB891562	AACGCCAUUAUCACACUAAAUA	138–117	0	22	60	63.33	−25.3	1.10
	mmu	BB891562	AAACGCCAUUAUCACACUAA	139–120	0	20	54	61.11	−24.2	1.15
miR-548aa	hsa	BB883545	AAAAACCACAAUUACUUUUGCACCA	592–568	0	25	67	64.18	−29.9	1.20
miR-548d-5p	hsa	BB883545	AAAAGUAAUUGUGGUUUUUGCC	574–595	1	22	55	70.91	−25.1	1.57
miR-548d-3p	mml	BB883545	CAAAAACCACAAUUACUUUUGC	593–572	1	22	57	68.42	−27.2	1.51
miR-675	hsa	DC648667	UGGUGCGGAGAGGGCCCACAGUG	562–584	0	23	55	38.18	−29.2	0.86
miR-675^*	hsa	DC648667	CUGUAUGCCCUCACCGCUCA	597–616	0	20	55	38.18	−29.2	0.86
miR-3591	rno	BB891562	AACACCAUUGUCACACUCCA	155–174	0	20	57	62.50	−23.6	1.12

NM: nucleotide mismatch; LM: length of mature miRNA; LP: length of miRNA precursor; MFE: minimum free energy; MFEI: minimum free energy index.