Tackling Skeletal Muscle Cells Epigenome in the Next-Generation Sequencing Era

Figure 1

During development and muscle regeneration, satellite cells exit quiescence, actively proliferate, and terminally differentiate and fuse to form new muscle fibers. Within the nucleus, these steps are finely tuned by modifications in high-order chromatin structures and nucleosome accessibility, by changes in protein complexes recruited at regulatory regions, by alterations in histones marks and in the DNA methylation state. NGS offers invaluable means to explore each of these variations genomewide and to accurately identify pathways and regulatory networks underlying satellite cells activation and differentiation.