Research Article

Development of Transgenic Papaya through Agrobacterium-Mediated Transformation

Table 1

Transformation efficiency and GUS activities of hypocotyls and immature zygotic embryos of Carica papaya cv. Shahi and Carica papaya cv. Ranchi infected by Agrobacterium tumefaciens strains LBA-4404.

TreatmentSurvivability (%)
(Mean ± SE)
Callus formation (%)
(Mean ± SE)
No. of GUS positive response (%)
(Mean ± SE)
No. of somatic embryos produced/explants
(Mean ± SE)
No. of regenerated plantlets/explants
(Mean ± SE)

C. papaya cv. Shahi
 Hypocotyls6.83 ± 0.26 42.41 ± 2.72 27.48 ± 1.408.41 ± 0.655.36 ± 0.47
 Zygotic embryos5.50 ± 0.2134.46 ± 1.12 20.06 ± 0.936.53 ± 0.603.38 ± 0.60
C. papaya cv. Ranchi
 Hypocotyls4.73 ± 0.3238.80 ± 0.7922.80 ± 1.507.60 ± 0.494.06 ± 0.35
 Zygotic embryos4.50 ± 0.1528.90 ± 1.6428.90 ± 1.644.43 ± 0.452.11 ± 0.17