The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori)
Figure 3
The expression analysis of BmBras2 during different silkworm developmental stages. (a) The expression levels of BmBras2 by western blotting; 1, fifth instar larvae; 2, pupae; 3, moth; 4, egg; 5, purified rBmBras2. (b) The relative expression levels of BmBras2 analyzed by real-time PCR. The relative expression level was calculated by using , where = (CT, BmBras2-CT, 18S rRNA) for different stages and (CT, BmBras2-CT, 18S rRNA) pupae.
