DUSP1 Gene Polymorphisms Are Associated with Obesity-Related Metabolic Complications among Severely Obese Patients and Impact on Gene Methylation and Expression

Table 5

Association of phenotype-associated SNPs with gene methylation levels. P values for significant associations and trends toward an association between relevant SNPs and CpG sites’ methylation are shown.

(a)

SNP	Localization	cg22473727	cg16957313	cg08452061	cg09493150	cg21121138	cg09799633	cg19537645	cg14968860
		Promoter	Promoter	Promoter	Promoter	Promoter	Promoter	Promoter	Promoter
rs13184134	Promoter	0.30	0.20	0.49	0.16	0.31	0.31	𝟎.𝟎𝟐^a	0.77
rs881150	Promoter	0.34	0.28	0.06	𝟎.𝟎𝟒^a	0.06	𝟎.𝟎𝟓^b	𝟎.𝟎𝟏^b	𝟎.𝟎𝟑^a
rs7702178	Intron 2	0.06	0.07	0.16	𝟎.𝟎𝟒^a	0.06	0.22	0.09	0.49

(b)

SNP	Localization	cg23002268	cg25108022	cg02352687	cg18121420	cg04525476	cg11757894	cg08293091
		Promoter	Promoter	Promoter	Promoter	Promoter	Promoter	Intron 1
rs13184134	Promoter	0.74	0.82	0.61	0.53	0.91	0.67	𝟎.𝟎𝟑^a
rs881150	Promoter	𝟎.𝟎𝟏^b,c	𝟎.𝟎𝟎𝟔^b,c	𝟎.𝟎𝟏^b	𝟎.𝟎𝟐^b	𝟎.𝟎𝟎𝟐^a,b,c	0.06	0.78
rs7702178	Intron 2	0.95	0.96	0.62	0.83	0.83	0.87	0.06

Boldface values represent statistically significant results (𝑃≤0.05).
aHeterozygote genotype demonstrates higher methylation levels versus common homozygote genotype.
bRare homozygote genotype demonstrates higher methylation levels versus common homozygote genotype.
cRare homozygote genotype demonstrates higher methylation levels versus heterozygote genotype.