Identification of Salt-Stress-Induced Genes from the RNA-Seq Data of Reaumuria trigyna Using Differential-Display Reverse Transcription PCR

Figure 1

Representative 6% denaturing polyacrylamide gel from a differential-display experiment. Differences between control and NaCl-treated tissues detected by DDRT-PCR with arbitrary primers (1, 2, 3, 4, 5, 6, and 8 in Table 1) in combination with the anchor primer II (Table 1) are shown. II-1, II-2, II-3, II-4, II-5, II-6, and II-8 represent the primer combinations. Lanes C and T contain the PCR products from the control and treated samples, respectively. Lane M contains a 100-bp DNA ladder marker. Arrows indicate the recovered differentially expressed cDNA fragments. Arbitrary primer 7 (Table 1) failed to generate any fragment length polymorphisms.