In Silico Prediction of T and B Cell Epitopes of Der f 25 in Dermatophagoides farinae

Table 1

Parameters used for proteins structural assessment.

Protein	Structural assessment methods	Ramachandran plot (%)	𝐺-factor	MCBL (%)	CBA (%)	𝑍-score	𝑄 value	RMSD
Der f 25	PROCHECK analysis	93.0^E	0.10^I	100.0	99.0
		7.0^F	0.42^J
		0.0^G	0.23^K
		0.0^H
	ProSa					−10.1
	QMEAN						0.772
	RMSD							0^L
2I9E	PROCHECK analysis	93.4%^E	0.25^I	100.0	98.9
		6.6%^F	0.55^J
		0.0^G	0.37^K
		0.0^H
	ProSa					−10.24
	QMEAN						0.798
	RMSD							0.056^L

MCBL: distribution of the main chain bond lengths; CBA: distribution of the covalent bond angles.
^EResidues in favorable regions; ^Fresidues in allowed regions; ^Gresidues in generally allowed regions; ^Hresidues in disallowed regions; ^I𝐺-factor score of the dihedral bonds; ^J𝐺-factor score of the covalent bonds; ^Koverall 𝐺-factor score; ^Lroot mean square deviation between C𝛼 Der f 25 structure and 2I9E template.