Transcriptomes That Confer to Plant Defense against Powdery Mildew Disease in Lagerstroemia indica
Table 3
Relative quantification and fold expression of differentially expressed genes in powdery mildew resistant “Natchez” (NAT) versus susceptible “Carolina Beauty” (CAB) cultivars.
Primer
Query name
NAT cDNA Ct (mean)
CAB cDNA Ct (mean)
Fold diff. (2-Nat-CabCt)
Fold diff. (2-Cab-NatCt)
Fold diff. (2-Nat-CabCt)
Fold diff. (2-Cab-NatCt)
Fold diff. (2-Nat-CabCt)
Fold diff. (2-Cab-NatCt)
Mean fold
cab-h1
B.9985
27.8
26.78
cab-h8
B.8346
27.83
28.75
nat-h1
T.9454
29.40
29.30
cab-rt1
B.7344
32.18
30.94
—
1.16
—
4.47
—
2.20
2.61
cab-rt2
B.2282
30.19
29.92
—
0.59
—
2.28
—
1.13
1.33
cab-rt3
B.3011
31.50
33.31
—
0.14
—
0.54
—
0.27
0.32
cab-rt5
B.18652
29.02
26.48
—
2.87
—
11.00
—
5.43
6.43
cab-rt6
B.21408
35.94
37.44
—
0.17
—
0.67
—
0.33
0.39
cab-rt7
B.21856
35.16
33.60
—
1.45
—
5.58
—
2.75
3.26
cab-rt8
B.22624
33.80
28.64
—
17.63
—
67.65
—
0.17
28.48
cab-rt9
B.22625
29.36
26.84
—
2.83
—
10.85
—
5.35
6.34
cab-rt10
B.23059
32.30
34.69
—
0.09
—
0.36
—
0.18
0.21
nat-rt6
T.13188
32.75
37.73
0.20
—
0.05
—
0.11
—
0.12
nat-rt8
T.13192
28.34
29.43
4.23
—
1.10
—
2.23
—
2.52
nat-rt9
T.13486
32.08
29.40
0.21
—
0.05
—
0.11
—
0.12
nat-rt11
T.17520
37.91
28.78
1.79
—
0.47
—
0.95
—
1.07
nat-rt13
T.8775
28.14
27.46
1.27
—
0.33
—
0.67
—
0.75
nat-rt14
T.9193
29.82
31.05
4.76
—
1.24
—
2.51
—
2.84
nat-rt15
T.17643
30.33
28.44
0.55
—
0.14
—
0.27
—
0.32
Note. We calculated the fold increase in expression for the NAT primers by treating NAT as the treatment and housekeeping gene as the control and compared to CAB treatment (). For the CAB primers we used CAB as the treatment and housekeeping gene as the control and compared to NAT treatment (). Highlight in bold (cab-h1), italic (cab-h8), and bold italic (nat-h1) indicates that fold difference of expression was calculated based on individual housekeeping gene. Mean fold was calculated from three housekeeping genes.