A Classification of Basic Helix-Loop-Helix Transcription Factors of Soybean

Figure 1

Alignment of the bHLH domain from 319 soybean proteins. The bHLH region was identified using a HLH hidden Markov model and trimmed or extended from soybean predicted proteins to fit the canonical region aligned in this figure and then aligned using a fast Fourier transform algorithm. A color key is used for the 20 amino acid residues. Extent of conservation and a consensus sequence are shown at top left. The basic and helix-loop-helix regions can be seen via the annotations and the color key. The consensus graph indicates 100% conserved residues as green (there are none in this alignment), 30% or more conserved residues as yellow, and less than 30% conserved residues as red. A full text version is available in Additional File 3.