MicroRNA and Putative Target Discoveries in Chrysanthemum Polyploidy Breeding

Figure 5

Validation of target genes in chrysanthemum embryos by qRT-PCR. The orange histogram represented the qRT-PCR validation, and the value of relative expression was shown on the left of y-axis. The blue slope lines represented the FPKM of each unigene obtained using RNA-Seq [19], and the value of FPKM was shown on the right of y-axis. NE12, NE18, and AE18 mean the normal embryo at 12 DAP, normal embryo at 18 DAP, and abnormal embryo at 18 DAP, respectively.