Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development

Figure 4

Validation of the gene-specific markers in different white and orange cultivars and F₂ populations. (a) The amplified fragments by the Bror-intron 1-F/R primers in 14-401 and 14-490. The blue lines indicate where the Bror-intron 1-F/R primers bind. The red arrows represent the boundaries of the exon/intron. (b) Validation of the gene-specific marker Bror-intron 1 in the parents 14-401 and 14-490 and its F₂ individuals. Among the F₂ individuals, 1–11 are the lines with the white inner leaves, and 12–19 are the lines with the orange inner leaves. (c) Validation of the gene-specific marker Bror-intron 1 in the breeding lines. 1–11 are 663, 1466, 1469, 1492, 1505, 1510, 1720, Hanxiu, Jindianchunwang, Kaichun, and Ribenxiayang with the white or yellow inner leaves. 12–23 are 1480, 14-102, 14-245, 14-253, 14-257, 14-277, 14-426, 14-662, 14-669, Changyanjubao, Shenmengjuhongxin, and Shenshijuhongxin with the orange inner leaves.

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