Differential Expression Profiling of Long Noncoding RNA and mRNA during Osteoblast Differentiation in Mouse

Figure 1

Global expression patterns of genes at the nine time points of osteoblast differentiation. (a) Hierarchical clustering of the transcriptome over the time period of osteoblast differentiation. All known genes with RPKM values ≥ 3 were used for analysis. (b) Number of genes showing up- or downregulation during osteoblast differentiation (fold change ≥ 2 or ≤0.5, FDR < 0.05). (c) Volcano plot showing differentially expressed genes in red and blue. The x- and y-axes represent the magnitude of fold changes (log₂ transformed) and adjusted value (−log₂) by Benjamini-Hochberg correction, respectively. FC = fold change (d18/d2). (d) Principal component analysis (PCA) of 4058 time series gene expression profiles for different samples (three replicates at each time point of osteoblast differentiation).

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