Hepatitis C Virus E2 Protein Ectodomain Is Essential for Assembly of Infectious Virions
Figure 6
Sedimentation equilibrium analysis. Buoyant density profile of S6.1/JFH1 (a) and S6.1/Con1E2 (b) cell lysates determined by equilibrium ultracentrifugation in sucrose gradient is reported on the top panel. Fractions of the 20%–60% gradient were collected from the top, and infectivity was determined by serial dilution and immunofluorescence staining anticore protein. The infectivity (pfu/ml) is shown as a bar chart. The density (g/ml) of each fraction is shown as a dotted line. Each sucrose fraction was processed by Western blot analysis as reported below the corresponding chart. The mouse monoclonal MMM33 anti-NS3 was used to detect the HCV nonstructural protein NS3, E1 and E2 proteins were revealed by the polyclonal Ch-L559 antisera and core protein was detected by the mouse monoclonal antibody 3G1-1.
