Novel Approach to Bile Duct Damage in Primary Biliary Cirrhosis: Participation of Cellular Senescence and Autophagy

Figure 2

Double immunostaining for senescence markers (p16^INK4a or p21^WAF1/Cip1) and cell cycle markers (G1-phase, cyclin D; S-phase, cyclin A) (red) and NCAM (green) in PBC, stage 4. (a), (c) The expression of senescent markers p16^INK4a and p21^WAF1/Cip1 is seen in NCAM-positive ductular cells (arrows) in PBC, stage 4. (b), (d) Most NCAM-positive ductular cells (arrows) express cyclin D, whereas there is no cyclin A expression in DRs in PBC, stage 4. Original magnification ×400.

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