A Crucial Role of Bone Morphogenetic Protein Signaling in the Wound Healing Response in Acute Liver Injury Induced by Carbon Tetrachloride
Figure 2
Generation of Bmpr1a knockout mouse with Cre recombination system (a) β-galactosidase staining of liver from Ad-LacZ-infected mouse. Scale bars: 50 μm. (b) Left panel: schematic illustration of floxed Bmpr1a gene and generation of deletion. Right panel: genomic PCR. Genomic DNA was obtained from liver (L) and brain (B) at 14 days postinfection (Ad-Cre +). PCR was performed using primer set: 5′-GGTTTGGATCTTAACCTTAGG (Fx1)/5′-TGGCTACAATTTGTCTCATGC (Fx4). (c) Left panel: schematic illustration of transcripts from and genes generated by Cre recombination. Right panel: total RNA was obtained from the liver at 14 days postinfection with Ad-Cre. RT-PCR for BMPR1A transcripts was carried out with primer sets: 5′-GAAAGCAGCAGGTGAAAGTC (Primer-for)/5′-CTATAATGGCAAAGCAATGG (Primer-rev). RT-PCR of GAPDH mRNA was performed as a control. (d) Imunofluorescence of Bmpr1a in the livers from mock- Ad-LacZ- and Ad-Cre-infected mice. Strong signals without nuclei were derived from erythrocytes Scale bars: 25 μm.
