Regulation of the G1/S Transition in Hepatocytes: Involvement of the Cyclin-Dependent Kinase Cdk1 in the DNA Replication
Figure 1
Sections of mouse liver evidencing detection of DNA replication and G2 phase. Mice were hepatectomized, injected at 46 hours after hepatectomy with BrdU, and killed 2 hours later (at 48 h). Livers were fixed for histological studies and detection of BrdU to visualize hepatocytes replicating DNA (a) or phosphohistone H3 (b) to detect cells in G2 phase. (a) This low magnification picture shows the detection of BrdU positive cells replicating DNA, illustrating that replicating hepatocytes are initially localized in the vicinity of the portal vein while around centrolobular veins (CV) only few hepatocytes replicate DNA at 48 h. (b) A higher magnification picture shows nuclei of hepatocytes reaching G2 phase (detection of phosphohistone H3 positive cells with punctuated nuclear signal: G2 and mitosis (M). Bars: 100 μm.
