Combined Stimulation with the Tumor Necrosis Factor
and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices
Figure 2
ATP content, LDH release, and expression of liver specific proteins. ATP content, expressed in nanomols/g of wet slices weight (a) and LDH release (b) were measured during the preincubation (Sl) and at the indicated times of culture. LDH release was also measured in primary culture of isolated hepatocytes (dark triangles). Western blot analysis of liver specific proteins (c) in normal liver (NL), in core before slicing (c), freshly prepared slice (Sl), after the preincubation (T0), and in culture at different times. Specific antibodies detecting albumin (Alb), transferrin (Trf), cytochrome P450 (CYP) 3A1/2, 2B, 2E1 subunits, and glutathione S-transferases (GST) A1 and P1 isoforms, were used. The western blot of HSC70 indicated that equal amounts of proteins were loaded in each lane. These experiments were performed on 2 independent experiments with 3 slices in each experiment. treatment versus control.
