Combined Stimulation with the Tumor Necrosis Factor
and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices
Figure 4
Caspase activities and LDH release in cultured slices and hepatocytes upon treatments with TGFβ, TNFα, and cycloheximide. DEVD- (a), IETD- (b), LEHD-AMC (c), caspase activities, and LDH release (d) in cultured slices at 24 and 48 h in control (Ctl) after treatments with TGFβ, cycloheximide, TNFα, and TNFα + cycloheximide. For slices treated with both TNFα and cycloheximide, caspase activities at 48 h were not presented because of a complete loss of viability between 24 and 48 h. Caspase activities and LDH release in liver slices are the results of 3 independent experiments with 2 or 3 slices in each experiment. treatment versus control.
