Combined Stimulation with the Tumor Necrosis Factor 𝜶 and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices

Figure 7

In situ immunodetection of Ki67 and phosphohistone H3. In situ immunostaining of Ki67 (Figures 7(a–c)) and phosphorylated histone H3 (Figures 7(d–f)) at 48 h of culture in untreated slices (a–d) and after EGF (b–e) or TNFα + EGF (c–f) treatments. Arrows indicate Ki67 or phosphohistone H3 positive cells. Bar: 100 μm.