Lipid Droplet Accumulation and Impaired Fat Efflux in Polarized Hepatic Cells: Consequences of Ethanol Metabolism
Figure 5
Adipophilin (ADRP) protein content and mRNA expression in WIF-B cells. WIF-B cells were cultured for 48 hours in the absence (control media, C) or presence of 25 mM ethanol (E25) with or without the presence of 0.5 mM oleic acid (O and E25 + O). (a) Representative Western blot indicating the presence of ADRP and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the loading control. (b) Relative content of adipophilin protein expressed as percent of control from four independent experiments. (c) Adipophilin mRNA expression detected in the WIF-B cells. *Significantly different () from control group and E25 group. #Significantly different from all other groups (C, E25, and O).
