Demonstration of Hepatitis C Virus RNA with In Situ Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver

Figure 1

HCV-RNA localization with LNA-based ISH in the liver of chimeric mice infected with HCV of different genotypes. (a)–(c) HCV noninfected, (d)–(f) HCV genotype 1a-infected, (g)–(i) HCV genotype 1b-infected, (j)–(l) HCV genotype 2a-infected, ((a), (d), (g), (j)) HE stain, ((b), (e), (h), (k)) immunostaining for human-specific CK8/18, ((c), (f), (i), (l)) LNA-based ISH, (*) mouse hepatocytes, and (**) human hepatocytes. HCV-RNA is demonstrated diffusely in the cytoplasm of human hepatocytes in the chimeric livers. No signals are seen in the noninfected liver.

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