Demonstration of Hepatitis C Virus RNA with In Situ Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver
Figure 3
HCV-RNA localization with LNA-based ISH in FFPE biopsy or surgical specimens of the liver. ((a), (b)) Case 10 with chronic hepatitis C, ((c), (d)) case 11 with chronic hepatitis C, ((e), (f)) case 15 with chronic hepatitis B (noncancerous portion of a surgical case for HCC), ((g), (h)) case 13 with fatty liver, ((i), (j)) case 16 with surgically removed hepatitis-C-related HCC, ((a), (c), (e), (g), (i)) HE stain, and ((b), (d), (f), (h), (j)) LNA-based ISH. In HCV-infected human livers, the HCV-RNA signals are demonstrated in a focal/partial pattern (b) or in a diffuse pattern (d). Signals are not demonstrated in the hepatocytes of HBV-infected liver (f), fatty liver (h), and hepatitis-C-related HCC (j). Nonspecific binding of the reagent to Kupffer cells and lymphoid cells is occasionally observed (arrows in the panels (b), (d), (f), and (h)).
