Demonstration of Hepatitis C Virus RNA with In Situ Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver

Table 1

HCV-RNA demonstration with LNA-based ISH and nested RT-PCR in FFPE human liver tissues.

Patient	Biopsy or; surgery	Diagnosis; New Inuyama Classification	Serum		FFPE liver tissue
			HCV-RNA (KIU/mL)	Nested RT-PCR	Nested RT-PCR	ISH
Case  1	B	Chronic hepatitis C, A2F2	5,1 × 10^2≦	1b	1b	+	Focal/partial
Case  2	B	Chronic hepatitis C, A1F1	2,8 × 10^2	1b	1b	+	Focal/partial
Case  3	B	Chronic hepatitis C, A2,F1	2,4 × 10^2	2a	2a	+	Diffuse
Case  4	B	Chronic hepatitis C, A1F0	1,9 × 10^2	2a	2a	−
Case  5	B	Chronic hepatitis C, A1F1	2,5 × 10^2	1b	1b	+	Focal/partial
Case  6	B	Chronic hepatitis C, A2F3	2,7 × 10^2	1b	−	−
Case  7	B	Chronic hepatitis C, A2F1	2,2 × 10^2	2a	−	+	Focal/partial
Case  8	B	Chronic hepatitis C, A3F3	5,1 × 10^2	1b	1b	+	Focal/partial
Case  9	B	Chronic hepatitis C, A2F1	2,9 × 10^2	1b	1b	+	Diffuse
Case  10	B	Chronic hepatitis C, A2F2	3,1 × 10^2	2a	−	+	Focal/partial
Case  11	B	Chronic hepatitis C, A2F3	3,3 × 10^2	1b	1b	+	Diffuse
Case  12	B	Chronic hepatitis B, A3F3	Not detected	Not done	−	−
Case  13	B	Fatty liver, A0F0	Not detected	Not done	−	−
Case  14	B	Autoimmune hepatitis, A3F3	Not detected	Not done	−	−
Case  15	S	Hepatitis-B-related HCC; Chronic hepatitis B, A2F2	Not detected	Not done	−	−; −
Case  16	S	Hepatitis-C-related HCC	84	Not done	−	−

New Inuyama Classification (1996).
Activity of inflammation: A1: mild, A2: moderate, and A3: severe.
Fibrosis: F0: none, F1: portal widening, F2: bridging fibrosis, and F3: bridging fibrosis with lobular distortion.