Review Article
Implication for Bone Marrow Derived Stem Cells in Hepatocyte Regeneration after Orthotopic Liver Transplantation
Table 1
Selected clinical studies.
| | Reference |
Pat. no. | Type of TX | D/R combination | Cases of hepatocyte chimerism | Hepatocyte chimerism % | Method | Additional findings |
| | Alison et al. 2000 [14] | 11
9 | OLT
HSCT | F→M
M→F | Not specified | 0.5–2% | FISH | Clonal growth of BM-HSC derived hepatocytes | | Theise et al. 2000 [15] | 4
2 | OLT
HSCT | F→M
M→F | 6/6 | 1–8%
(4–43% adjusted) | FISH | Distribution of chimeric hepatocytes suggests different pathways of hepatic BM-HSC engraftment | |
Körbling et al. 2002 [16] | 6 | HSCT | M→ F | 4/6 | 4–7% | FISH | Chimerism is not correlated with GVHD related tissue damage | | Fogt et al. 2002 [17] | 13 | OLT | | none | 0% | FISH | Hepatocyte chimerism is non frequent event in OLT | | Kleeberger et al. 2002 [18] | 9 | OLT | Not specified | 7/9 | Not specified | STR | High frequency of cholangiocyte chimerism; hepatocyte chimerism associated with HCV recurrence | | Wu et al. 2003 [19] | 7
6 | OLT
Pediatric OLT | F→M | none | <0.4% putative hepatocytes | FISH |
Recipient parenchymal cells are rare to nonexistent | |
ten Hove et al. 2003 [20] | 5
11 | OLT | F→M
HLA I mismatch | 1/5
Not specified | Not specified | FISH
HLA-IH | Common endothelial and bile duct epithelial cell chimerism | | Ng et al. 2003 [21] | 10
5
2 | OLT | F→M
M→ F
No sex mismatch | 6/10
Not specified | <0.62%
Not specified | FISH
STR | High frequency of hepatic chimerism in Kupffer cells and macrophages | | Idilman et al. 2004 [22] | 11
5 | OLT | F→M
M→ F | 6/5
5/5 | 0–2.4%
1.6–3.3% | FISH | BM-HSC derived hepatocytes are more common early after OLT, chimerism is not related to ACR | | Idilman et al. 2007 [23] | 9 | OLT | | 9/9 | 0.05–3.2% | FISH | BM-HSC derived hepatocyte repopulation is an early event | | Pilat et al. 2012 [24] | 14 | OLT | F→M | none | 0% | FISH | BM-HSC derived hepatocyte repopulation is not of clinical relevance | | Aini et al. 2013 [25] | 24 | Pediatric OLT | Not specified | 12/24 | 2.5–3.4% | FISH
STR | Hepatocyte chimerism is suggested to be a common event and not correlated with hepatic injury |
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OLT: orthotopic liver transplantation, HSCT: hematopoietic stem cell transplantation, F: female, M: male, FISH: fluorescent in-situ hybridization, STR: short tandem repeat/microsatellite analysis, GVHD: graft-versus-host disease, HLA-IH: HLA specific immunohistochemistry, HCV: hepatitis C virus, and ACR: acute cellular rejection.
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