Histological Characterization of Biliary Intraepithelial Neoplasia with respect to Pancreatic Intraepithelial Neoplasia

Table 1

Primary antibodies used for immunohistochemical analysis.

Antigen	Clone	Company	Dilution	Antigen retrieval
MUC1	DF3	Toray Fuji Bionics (Tokyo, Japan)	1 : 50	MW
MUC2	Ccp58	Novocastra (Newcastle, UK)	1 : 100	MW
MUC5AC	CLH2	Novocastra	1 : 200	MW
Cytokeratin 20	Ks 20.8	DakoCytomation (Glostrup, Denmark)	1 : 50	MW
Cyclin D1	SP4	Nichirei (Tokyo, Japan)	Prediluted	MW*
p21	EPR3993	Abcam (Cambridge, UK)	1 : 100	MW
p53	DO-7	DakoCytomation	1 : 100	MW
S100P	EPR6143	Abcam	1 : 100	MW

MW: microwaving in 10 nmol/L citrate buffer (pH 6.0) for 20 minutes; MW*: microwaving in tris-ethylenediaminetetraacetic acid buffer (pH 9.0) for 20 minutes.