Role of Tumor Associated Fibroblasts in Human Liver Regeneration, Cirrhosis, and Cancer
Figure 6
L-MASCs cultured in the presence of tumor-conditioned medium modify their growth characteristics. (a–d) Anchorage independent growth (soft agar assay). Phase contrast images of the colonies formed by L-MASCs grown in non-CM (a), HepG2-CM (b), and Huh7-CM (c). Scale bar = 100 μm. Results are expressed as mean ± S.D. *, ** versus non-CM and HepG2-CM, respectively (d). (e–f) Migration ability (scratch assay). Phase contrast images of three scratches produced in L-MASCs grown in non-CM, HepG2-CM, and Huh7-CM, respectively, taken at different time points. Scale bar = 50 μm. Results are expressed as mean ± S.D. *, ** versus non-CM and HepG2-CM, respectively (f).
