Investigation of Homocysteine-Pathway-Related Variants in Essential Hypertension

Table 1

SNP and assay information.

Gene	Location	rs number	SNP	AA change	Assay	Primer sequence	Product size	Enzyme	Digest fragment
MTHFR	1p36.3	rs1801133	C677T	A222V	PCR-RFLP	FWD: 5′ TGAAGGAGAAGGTGTCTGCGGGA 3′	198 bp	HinfI	C-198 bp
						REV: 5′ AGGACGGTGCGGTGAGAGTG 3′			T-175 bp and 23 bp
MTHFR	1p36.3	rs1801131	A1298C	E429A	HRM	FWD: 5′ CTTTGGGGAGCTGAAGGACTACTAC 3′	163 bp	N/A	N/A
						REV: 5′ CACTTTGTGACCATTCCGGTTTG 3′
MTRR	5p15.31	rs1801394	A66G	I22M	HRM	FWD: 5′ GCAAAGGCCATCGCAGAAGACAT 3′	118 bp	N/A	N/A
						REV: 5′ AAACGGTAAAATCCACTGTAACGGC 3′
MTHFD1	14q24	rs2236225	G1958A	R653Q	HRM	FWD: 5′ CATTCCAATGTCTGCTCCAA 3′	254 bp	N/A	N/A
						REV: 5′ GTTTCCACAGGGCACTCC 3′

AA: amino acid; PCR-RFLP: polymerase chain reaction-restriction fragment length polymorphism; HRM: high resolution melt.