Research Article

Distinct Molecular Effects of Angiotensin II and Angiotensin III in Rat Astrocytes

Figure 3

Effect of AMA and GluP on Ang II- and Ang III-induced DNA synthesis. Quiescent monolayers of brainstem (a) and cerebellum (b) astrocytes were incubated for 48 hours with 100 nM Ang II or Ang III in the presence and absence of 10 μM AMA or 100 μM GluP. During the last 24 hours of treatment, 3H-Thymidine was added and DNA synthesis measured as described. Cells were also treated with the inhibitors alone. Each value represents the mean ± SEM of preparations of brainstem and cerebellum astrocytes from 6 or more litters of neonatal rat pups. *denotes that as compared to basal levels of DNA synthesis.
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