Strategies Aimed at Nox4 Oxidase Inhibition Employing Peptides from Nox4 B-Loop and C-Terminus and p22^phox N-Terminus: An Elusive Target

Figure 2

Examination of Nox4 C-terminal tail peptides as Nox4 inhibitors. (a) Map of overlapping Nox4 C-terminal region synthetic octa- and nonapeptides used to inhibit Nox4 activity. Numbers correspond to location and span of the N- and C-terminus for each peptide. (b) COS-Nox4 cell lysates were preincubated with various concentrations of C555–562, C560–567, C565–572, and C570–578 for 15 min at 24°C, and H₂O₂ was measured using Amplex Red. Data represent the mean ± SEM of 3 experiments. ^∗𝑃<0.05 indicates a significant difference between COS-22 and COS-Nox4 cell lysates. ^#𝑃<0.05 indicates a significant difference between COS-Nox4 + 0 μM peptide and COS-Nox4 + DPI.

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