Neurokinin-1 Receptor Antagonist Treatment in Polymicrobial Sepsis: Molecular Insights

Table 1

Primer sequences and optimal conditions used in PCR analysis.

Gene name	Sense primer sequence (5^′-3^′)	Antisense primer sequence (5^′-3^′)	Amplification conditions	No. of amplification cycles
NK-1R	CTT GCC TTT TGG AAC CGT GTG	CAC TGT CCT CAT TCTCTT GTGGG	95^∘C30s;59^∘C30s;72^∘C30s	38
NK-2R	TGC TGT CAT CTG GCT GGT AG	TCT TCC TCG GTT GGT GTC CC	95^∘C30s;61^∘C30s;72^∘C30s	42
18S	GTA ACC CGT TGA ACC CCA TT	CCA TCC AAT CGG TAG TAG CG	95^∘C30s;59^∘C30s;72^∘C30s	24