Myocardial neutrophil recruitment after I/R in MyD88−/− and Trif−/− mice. Twenty-four hours after 60 min of left anterior descending coronary artery (LAD) ligation, the hearts were isolated, perfused, and digested. After removal of the large cardiomyocytes through filtration, 50% of total cells were loaded onto flow cytometry and gated on Gr-1 and CXCR2. (a) Total Gr-1+ cells as measured by flow cytometry from the hearts subjected to I/R in MyD88−/− mice. Each error bar represents mean ± SD of 4 mice. A small number of neutrophils were recovered from the sham-operated hearts as indicated by the line. (b) A representative example of flow cytometry plots of myocardial infiltrating cells from sham, WT-I/R, and MyD88−/−-I/R mice. (c) Total Gr-1+ cells as measured by flow cytometry from the hearts subjected to I/R in Trif−/− mice. Each error bar represents mean ± SD of 3 mice. A small number of neutrophils were recovered from the sham-operated hearts as indicated by the line. (d) A representative example of flow cytometry plots of myocardial infiltrating cells from WT-I/R and Trif−/−-I/R mice. FSC, forward scatter; SSC, side scatter. (Feng et al., [100], used with permission).
