Long-Lasting Effect of Infant Rats Endotoxemia on Heat Shock Protein 60 in the Pancreatic Acinar Cells: Involvement of Toll-Like Receptor 4
Figure 2
Western blot analysis of TLR4 protein level in the adult rat pancreatic acinar cells under basal conditions (lane 1), stimulated in vitro by caerulein at the dose of 10−8 M (lane 2), treated in the infant animals by lipopolysaccharide (Escherichia coli) at the doses of 5 mg/kg/day × 5 days + caerulein 10−8 M (lane 3), 10 mg/kg/day × 5 days + caerulein 10−8 M (lane 4), and 15 mg/kg/day × 5 days + caerulein 10−8 M (lane 5) after 5 hours of incubation. Cross indicates significant () change, as compared to the control group. Asterisk indicates significant () change, as compared to the value obtained from the rats treated by increasing doses of LPS (10 or 15 mg/kg/day × 5 days) in combination with caerulein (10−8 M), as compared to the caerulein (10−8 M) alone stimulation. The blots were stripped and probed with GAPDH to document equal protein loading. All presented results were obtained in 4 consecutive experiments and are representative for the observed phenomenon.