Cytosolic Double-Stranded DNA as a Damage-Associated Molecular Pattern Induces the Inflammatory Response in Rat Pancreatic Stellate Cells: A Plausible Mechanism for Tissue Injury-Associated Pancreatitis
Figure 2
Transcription of cytokine and chemokine mRNA was induced by synthetic double-stranded DNA (dsDNA). (a) Extracellular DNA stimulation had no effect on inflammatory cytokines and chemokines, such as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), monocyte chemoattractant protein-1 (MCP-1), and cytokine-induced neutrophil chemoattractant 1 (CINC-1). In contrast, intracellular dsDNA (at 10 g/mL) had stimulatory effects on their expression, including the expression of their transcriptional factors nuclear factor-kappa B (NF-κB) and reticuloendotheliosis viral oncogene homolog B (RelB) (b). Release of MCP-1 was also confirmed by ELISA (c). PSCs: pancreatic stellate cells, TFx: + transfection reagent lipofectamine. *, **.
