Rapamycin Augments the NMDA-Mediated TNF Suppression of MRSA-Stimulated RAW264.7 Murine Macrophages
Figure 2
(a) Effects of rapamycin on RAW264.7 murine macrophages stimulated by MW2 MRSA. The MW2 (USA400) MRSA strain stimulated more TNF secretion by RAW264.7 murine macrophages when exposed to rapamycin at concentrations of 0.1 and 1 ng/mL and less when exposed to rapamycin at 10 and 100 ng/mL. The response at 0.1 ng/mL (second column) and 10 ng/mL (fourth column) represented an increase of 13% and a decrease of 11%, both significantly different () from the untreated control (first column). The responses at 1 ng/mL (third column) and 100 ng/mL (fifth column) were an 8% increase and 4% decrease, which were not significantly different from the control. The results are depicted as fold of change compared to the control with 95% confidence intervals shown as “error bars.” . The control response was pg/mL. (b) Effects of rapamycin on RAW264.7 murine macrophages stimulated by LAC MRSA. The LAC (USA300) MRSA strain stimulated more TNF secretion by RAW264.7 murine macrophages when exposed to rapamycin at 0.1 and 1 ng/mL and less when exposed to rapamycin at 10 and 100 ng/mL. The response at 0.1 ng/mL (second column) had an increase of 14%, significantly different () from the untreated control (first column). The response at 1 ng/mL (third column) presented an increase of 9%, which did not reach statistical significance. The responses at 10 ng/mL (fourth column) and 100 ng/mL (fifth column) demonstrated decreases of 11% and 22%—significantly different from the control at . The results are depicted as fold of change compared to the control with 95% confidence intervals shown as “error bars.” . The control response was 27,965 pg/mL ± 699 pg/mL.
