Infiltration of Proinflammatory M1 Macrophages into the Outer Retina Precedes Damage in a Mouse Model of Age-Related Macular Degeneration
Figure 2
Quantification of retinal pathology and macrophage infiltration in CEP-MSA-immunized mice. (a) Combined pathology score per section (all kinds of lesions and IPM-infiltrating cells). The significant changes due to CEP-MSA immunization occur at the early time point in the BALB/c background, whereas they occur at intermediate time points for B6 mice. (b) CEP-MSA immunization accelerates IPM-infiltrating cell recruitment at early stages of AMD development, prior to major retinal damage. Mean values are shown; error bars represent S.D. (*) denotes statistically significant differences (). Data from one representative experiment was used for this analysis; similar results were obtained in additional repeat experiments. (c) Haematoxylin & Eosin (H&E) staining of frozen sections confirmed the accelerated macrophage recruitment associated with CEP-MSA immunization. 5 sections were scored per mouse, with 3 mice per group at the intermediate time point (d 100–200 p.i.). Mean values are shown; error bars represent S.D.
