Research Article

The Effects of Insufflation Conditions on Rat Mesothelium

Table 1

Summary of light microscopy (LM) observations 12 h after insufflation with CO2 for 2 h at 5 mm/Hg.

Group 1
 ANAD
 BNAD
 CNAD
Group 2
 ANAD
 BInflammatory cell infiltrate (++)
Fragmentation and desquamation of mesothelium in some sections
 CInflammatory cell infiltrate (+++)
Some inflammatory cells migrated onto mesothelium surface
 DInflammatory cell infiltrate (+)
 EInflammatory cell infiltrate variable in concentration along mesothelium (+ to +++)
Inflammatory cells migrated onto mesothelium surface
Group 3
 AInflammatory cell infiltrate (+++) with cells on mesothelial surface
Loss of mesothelium in some sections of intense inflammatory cell infiltration
Remaining areas uninvolved
 BScant areas of inflammatory cell infiltrate (+)
 CInflammatory cell infiltrate (++)
Mesothelial cell nuclei rounded and projecting above mesothelial surface
 DScant areas of inflammatory cell infiltrate (+)
 EScant areas of inflammatory cell infiltrate (+)
Group 4
 APatches of inflammatory cell infiltrate (+)
 BInflammatory cell infiltrate (+ to ++)
 CPatches of inflammatory cell infiltrate (+ to +++)
 DPatches of inflammatory cell infiltrate (+)
 ENAD

Group 1: control animals, no treatment with CO2. Group 2: insufflation with room temperature, dry CO2. Group 3: insufflation with warm, dry CO2. Group 4: insufflation with warm, humidified CO2. Letters (A–E) refer to individual rats. NAD: no abnormality detected.