Prostaglandin E₂ Does Not Modulate CCR7 Expression and Functionality after Differentiation of Blood Monocytes into Macrophages

Figure 7

MDMM-1 cells and MDMs do not migrate toward the CCR7-specific ligands CCL19 and CCL21. (a) Chemotaxis assays using 300 ng/mL CCL19 and CCL21 were performed using MM1 and MDMM-1 cells incubated in the presence or absence of 1 μM PGE₂ for 24 h. Data represent the means of three different experiments. (b) Blood monocytes from five different healthy donors were differentiated into MDMs and used for chemotaxis assays as described. The mean number of spontaneously migrated cells was subtracted from the number of cells that migrated in response to chemokines. ^∗∗𝑃<0.01,  ^∗∗∗𝑃<0.001, and ^∗∗∗∗𝑃<0.0001.

(a)

(b)