Haptoglobin Genotype-Dependent Anti-Inflammatory Signaling in CD163⁺ Macrophages

Figure 1

CD163-dependent IL-10 secretion following scavenging of polymorphic Hp:Hb complexes. (a) Effect of increasing concentrations of polymorphic Hp:Hb complexes on IL-10 secretion by CD163⁺ human monocyte-derived macrophages at 24 h. (b) Timecourse of IL-10 secretion following addition of polymorphic Hp:Hb complexes at 1 mg/mL. (c) IL-10 secretion by CD163⁺ macrophages stimulated with Hb alone, Hp1-1, Hp2-2, or polymorphic Hp:Hb complexes (1 mg/mL) at 24 h. (d) Effect of a panel of anti-CD163 antibodies (20 𝜇g/mL) on IL-10 secretion induced by polymorphic Hb:Hp complexes (1 mg/mL) at 24 h. All IL-10 assays were carried out in duplicate, and results are expressed as mean IL-10 (pg/mL) ± SEM from 𝑛=3--9 experiments. ^∗𝑃<0.01.

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