Decline in the number of neutrophils infiltrating the bronchi and lungs induced by wogonin treatment. DO11.10 mice were nebulized with either OVA or PBS from days −2 to 0. Some mice received wogonin (2 mg/kg) or distilled water orally five times a week from days −7 to 0. On day 1, the mouse BALF was collected in order to count the number of total cells ((a), left) and neutrophils ((a), right). The mouse lungs were excised and subjected to hematoxylin and eosin staining (b). Histological staining was performed on the lungs removed from the mice that received water and were nebulized with PBS (left panel), the mice that received water and were nebulized with OVA (center panel), and the mice that received wogonin and were nebulized with OVA (right panel). Original magnification: ×200.
